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. 2006 Mar;44(3):854–856. doi: 10.1128/JCM.44.3.854-856.2006

TABLE 2.

Reactions for all media analyzed using software version VT2-R4.01

Test name Medium and identification resultsa
CBA
TSA
SBA
MAC
%P %N %E %P %N %E %P %N %E %P %N %E
Ala-Phe-Pro-arylamidase 0 99 1 2 98 0 0 100 0 0 100 0
Adonitol 0 100 0 0 100 0 0 100 0 0 100 0
l-Pyrrolydonyl-arylamidase 0 98 2 0 99 1 0 99 1 0 100 0
l-Arabitol 0 99 1 0 100 0 0 100 0 0 100 0
d-Cellobiose 2 98 0 2 98 0 2 98 0 2 97 1
Beta-galactosidase 0 100 0 0 100 0 0 100 0 0 100 0
H2S production 0 100 0 0 100 0 0 100 0 0 100 0
Beta-N-acetyl-glucosaminidase 84 15 1 80 15 5 83 14 3 52 35 13
Glutamyl arylamidase pNA 1 97 2 0 96 4 1 97 2 17 82 1
d-Glucose 100 0 0 100 0 0 100 0 0 100 0 0
Gamma-glutamyl-transferase 100 0 0 100 0 0 100 0 0 100 0 0
Fermentation/glucose 0 100 0 1 99 0 1 99 0 2 98 0
Beta-glucosidase 0 100 0 0 100 0 0 100 0 0 100 0
d-Maltose 27 72 1 11 87 2 24 76 0 6 92 2
d-Mannitol 94 2 4 88 4 8 96 2 2 96 4 0
d-Mannose 70 21 9 49 37 13 60 32 8 44 45 12
Beta-xylosidase 0 100 0 0 100 0 0 100 0 0 100 0
Beta-alanine arylamidase pNA 93 7 0 92 8 0 93 7 0 89 11 0
l-Proline arylamidase 57 40 3 92 7 1 38 61 1 12 80 8
Lipase 0 100 0 0 100 0 0 100 0 0 100 0
Palatinose 0 100 0 0 100 0 0 100 0 0 100 0
Tyrosine arylamidase 70 28 2 93 7 0 50 50 0 7 92 1
Urease 0 100 0 0 99 1 0 100 0 46 43 12
d-Sorbitol 92 4 4 84 7 9 93 4 3 92 3 5
Saccharose/sucrose 99 1 0 99 1 0 99 1 0 97 2 1
d-Tagatose 1 98 1 1 99 0 0 99 1 3 95 2
d-Trehalose 1 98 1 0 100 0 0 100 0 0 100 0
Citrate (sodium) 78 22 0 58 41 1 75 25 0 79 21 0
Malonate 31 45 24 6 83 11 36 43 21 71 25 4
5-Keto-d-gluconate 0 100 0 0 100 0 0 100 0 0 100 0
l-Lactate alkalinisation 100 0 0 100 0 0 100 0 0 100 0 0
Alpha-glucosidase 0 100 0 0 100 0 0 100 0 0 100 0
Succinate alkalinisation 99 1 0 98 2 0 99 1 0 100 0 0
Beta-N-acetyl-galosaminidase 68 29 3 48 44 7 68 31 1 64 35 1
Alpha-galactosidase 0 100 0 0 100 0 0 100 0 0 100 0
Phosphatase 39 30 31 14 62 24 44 31 25 91 2 7
Glyine arylamidase 0 99 1 0 100 0 0 99 1 0 98 2
Ornithine decarboxylase 0 100 0 0 100 0 0 100 0 0 100 0
Lysine decarboxylase 0 100 0 0 100 0 0 100 0 0 100 0
l-Histidine assimilation 10 86 4 8 92 0 14 84 2 4 94 2
Courmarate 74 26 0 71 29 0 77 23 0 64 36 0
Beta-glucoronidase 0 100 0 0 100 0 0 100 0 0 100 0
O/129 resistance (comp.vibrio) 82 18 0 71 28 1 78 21 1 66 33 1
Glu-Gly-Arg-arylamidase 0 100 0 0 100 0 0 100 0 0 100 0
l-Malate assimilation 20 78 2 13 86 1 22 77 1 6 93 1
Ellman 72 8 20 63 14 23 68 16 16 2 96 2
l-Lactate assimilation 11 86 3 8 89 3 11 87 2 3 95 2
a

Percent first choice for B. pseudomallei for Columbia horse blood agar (CBA), 81%; for Trypticase soy agar (TSA), 78%; for Columbia sheep blood agar (SBA), 75%; for MacConkey agar (MAC), 63%. %P, percentage of isolates positive; %N, percentage of isolates negative; %E, percentage of isolates equivocal.