TABLE 2.
Test name | Medium and identification resultsa
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CBA
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TSA
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SBA
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MAC
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%P | %N | %E | %P | %N | %E | %P | %N | %E | %P | %N | %E | |
Ala-Phe-Pro-arylamidase | 0 | 99 | 1 | 2 | 98 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
Adonitol | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
l-Pyrrolydonyl-arylamidase | 0 | 98 | 2 | 0 | 99 | 1 | 0 | 99 | 1 | 0 | 100 | 0 |
l-Arabitol | 0 | 99 | 1 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
d-Cellobiose | 2 | 98 | 0 | 2 | 98 | 0 | 2 | 98 | 0 | 2 | 97 | 1 |
Beta-galactosidase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
H2S production | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
Beta-N-acetyl-glucosaminidase | 84 | 15 | 1 | 80 | 15 | 5 | 83 | 14 | 3 | 52 | 35 | 13 |
Glutamyl arylamidase pNA | 1 | 97 | 2 | 0 | 96 | 4 | 1 | 97 | 2 | 17 | 82 | 1 |
d-Glucose | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 |
Gamma-glutamyl-transferase | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 |
Fermentation/glucose | 0 | 100 | 0 | 1 | 99 | 0 | 1 | 99 | 0 | 2 | 98 | 0 |
Beta-glucosidase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
d-Maltose | 27 | 72 | 1 | 11 | 87 | 2 | 24 | 76 | 0 | 6 | 92 | 2 |
d-Mannitol | 94 | 2 | 4 | 88 | 4 | 8 | 96 | 2 | 2 | 96 | 4 | 0 |
d-Mannose | 70 | 21 | 9 | 49 | 37 | 13 | 60 | 32 | 8 | 44 | 45 | 12 |
Beta-xylosidase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
Beta-alanine arylamidase pNA | 93 | 7 | 0 | 92 | 8 | 0 | 93 | 7 | 0 | 89 | 11 | 0 |
l-Proline arylamidase | 57 | 40 | 3 | 92 | 7 | 1 | 38 | 61 | 1 | 12 | 80 | 8 |
Lipase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
Palatinose | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
Tyrosine arylamidase | 70 | 28 | 2 | 93 | 7 | 0 | 50 | 50 | 0 | 7 | 92 | 1 |
Urease | 0 | 100 | 0 | 0 | 99 | 1 | 0 | 100 | 0 | 46 | 43 | 12 |
d-Sorbitol | 92 | 4 | 4 | 84 | 7 | 9 | 93 | 4 | 3 | 92 | 3 | 5 |
Saccharose/sucrose | 99 | 1 | 0 | 99 | 1 | 0 | 99 | 1 | 0 | 97 | 2 | 1 |
d-Tagatose | 1 | 98 | 1 | 1 | 99 | 0 | 0 | 99 | 1 | 3 | 95 | 2 |
d-Trehalose | 1 | 98 | 1 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
Citrate (sodium) | 78 | 22 | 0 | 58 | 41 | 1 | 75 | 25 | 0 | 79 | 21 | 0 |
Malonate | 31 | 45 | 24 | 6 | 83 | 11 | 36 | 43 | 21 | 71 | 25 | 4 |
5-Keto-d-gluconate | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
l-Lactate alkalinisation | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 |
Alpha-glucosidase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
Succinate alkalinisation | 99 | 1 | 0 | 98 | 2 | 0 | 99 | 1 | 0 | 100 | 0 | 0 |
Beta-N-acetyl-galosaminidase | 68 | 29 | 3 | 48 | 44 | 7 | 68 | 31 | 1 | 64 | 35 | 1 |
Alpha-galactosidase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
Phosphatase | 39 | 30 | 31 | 14 | 62 | 24 | 44 | 31 | 25 | 91 | 2 | 7 |
Glyine arylamidase | 0 | 99 | 1 | 0 | 100 | 0 | 0 | 99 | 1 | 0 | 98 | 2 |
Ornithine decarboxylase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
Lysine decarboxylase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
l-Histidine assimilation | 10 | 86 | 4 | 8 | 92 | 0 | 14 | 84 | 2 | 4 | 94 | 2 |
Courmarate | 74 | 26 | 0 | 71 | 29 | 0 | 77 | 23 | 0 | 64 | 36 | 0 |
Beta-glucoronidase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
O/129 resistance (comp.vibrio) | 82 | 18 | 0 | 71 | 28 | 1 | 78 | 21 | 1 | 66 | 33 | 1 |
Glu-Gly-Arg-arylamidase | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
l-Malate assimilation | 20 | 78 | 2 | 13 | 86 | 1 | 22 | 77 | 1 | 6 | 93 | 1 |
Ellman | 72 | 8 | 20 | 63 | 14 | 23 | 68 | 16 | 16 | 2 | 96 | 2 |
l-Lactate assimilation | 11 | 86 | 3 | 8 | 89 | 3 | 11 | 87 | 2 | 3 | 95 | 2 |
Percent first choice for B. pseudomallei for Columbia horse blood agar (CBA), 81%; for Trypticase soy agar (TSA), 78%; for Columbia sheep blood agar (SBA), 75%; for MacConkey agar (MAC), 63%. %P, percentage of isolates positive; %N, percentage of isolates negative; %E, percentage of isolates equivocal.