TABLE 1.

Primers for nested PCR of 18S rRNA gene in malaria parasites

Species	Primer	Sequence (5′-3′)	Size (bp) of PCR product
Plasmodium sp.	rPLU5	CCTGTTGTTGCCTTAAACTTC	1,100
	rPLU6	TTAAAATTGTTGCAGTTAAAACG
P. falciparum	rFAL1	TTAAACTGGTTTGGGAAAACC AAATATATT	205
	rFAL2	ACACAATGAACTCAATCATGA CTACCCGTC
P. vivax	rVIV1	CGCTTCTAGCTTAATCCACAT AACTGATAC	120
	rVIV2	ACTTCCAAGCCGAAGCAAAGA AAGTCCTTA
P. ovale	rOVA1	ATCTCTTTTGCTATTTTTTAG TATTGGAGA	800
	rOVA2	GGAAAAGGACACATTAATTGT ATCCTAGTG
P. malariae	rMAL1	ATAACATAGTTGTACGTTAAG AATAACCGC	144
	rMAL2	AAAATTCCCATGCATAAAAAA TTATACAAA