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TABLE 1.

PCR results for clinical isolates of Bordetella spp. used in this study

Bordetella species Strains Results of conventional IS481 PCR assay
B. pertussisa E161, E194, E144, D946 +
B. parapertussis B160, C518, B149, D568, E201
B. bronchisepticab A345, D445, D760, E010, E023
B. holmesiic C690, C692, C693, C694, D130 +
a

The IS481 gene is present at 80 to 100 copies in B. pertussis (9).

b

The IS481 gene is present as one copy in some strains of B. bronchiseptica (Gladbach et al., Abstr. 7th Int. Symp. Pertussis).

c

The IS481 gene is present at 8 to 10 copies in B. holmesii (5).