Skip to main content
. 2006 Mar;72(3):1793–1799. doi: 10.1128/AEM.72.3.1793-1799.2006

FIG. 2.

FIG. 2.

Genetic disruption of ZEA1 and ZEA2. (A) Schematic of disruption vectors integrated into the chromosome at the appropriate location and digested with BseRI (BR) and BsaBI (BB). Numbers indicate sizes of the predicted restriction fragments in kb. Genomic DNA, —; gene fragment used in disruption vector, ····; pHYG4, Created by potrace 1.16, written by Peter Selinger 2001-2019 Created by potrace 1.16, written by Peter Selinger 2001-2019 Created by potrace 1.16, written by Peter Selinger 2001-2019 . (B) Southern hybridization analysis of genomic DNA from the disrupted transformants (M1 and M2), ectopic transformants (E), and PH-1. Probes were generated with the respective gene fragment used in the disruption vector. The sizes (kilobases) of the fragments are shown on the right of each autoradiogram.