TABLE 1.
Primers used in this study
| Primer | Sequence |
|
|---|---|---|
| Forward (5′-3′) | Reverse (5′-3′) | |
| Gene disruption | ||
| ZEA1 | GCAATGCGTCCAGCTCCAAAAG | TCGGTTCACCTCGGTCAAATCCAG |
| ZEA2 | GGAGGATATGGGCGTGGTGAAGGA | GGCTGGCTGATCTCGGGCAAAGT |
| Sequencing intercontig region | ||
| 118A | GTGGCTGATCCCGCTCTTTGACG | TACAAGCATGGCGCAACAATAGAC |
| 118B | GACGGCCTCAACCTTTTCAGTG | TACAAGCATGGCGCAACAATAGAC |
| Expression analysis | ||
| ZEA1 | GAAGAGGCCCCGGTAGCGATAAC | TGAAGCCACTCCAGCAGCAGAATT |
| ZEA2 | GTCTCACTGACTTTGTTCGCAT | TCAAAGGATGTTCCTGGTTGCT |
| AUR1 | GGATTCACCGTGCCCGACAT | TTCAACGCAGAGCTCCATTACGC |
| Cluster analysis | ||
| Fg02393.1 | ACCATCCAATCTCGGGCTTT | TAACTTCAGTCGCCTCGCAAA |
| Fg02394.1 | TCGGGTCGAGGACTTCTGTG | GGTATGCTCCTGGACAGCGT |
| Fgd117-550 | CCAACGATTACACCAAGTCTCACTAT | GTTGTCTGGACGAGACAGGGTAC |
| Fg12015 | TGCTGGATAAGATTGCTCGTCTC | TCTCCAGTATCTGCTCCGGTCT |
| Fg12056 | AAATAATTTACCCGTTCTTCTGGGAACT | CTGAAACGGAGGTGTTGAGG |
| Fg02398.1 | GCTGTGGCCCAAATGCA | GCTATGATCGCCGATAGAAAGG |
| β-Tubulin | TCGACTGGATCCCCAACAAC | GAACTGGTCGTCGACACGCT |
| GAPDH | CTACATGCTCAAGTACGACTCTTCCC | GCCGGTCTCGGACCACTTG |
| ZEA1 | GGCACTTTGACAACCGCTTC | TTGCGCCGTCTGAGTACCC |
| ZEA2 | ACATGCGTGTCGTTGTTGTAAG | TGCACCGTGAGAAGCCGT |