TABLE 1.
Table of primersa
| Primer name | Base positionb | Sequence 5′→3′ |
|---|---|---|
| 8F | 8-27 | AGAGTTTGATCCTGGCTCAG |
| 8F-Bio | 8-27 | Bio-AGAGTTTGATCCTGGCTCAG |
| 27R | 27-8 | CTGAGCCAGGATCAAACTCT |
| 27R-Bio | 27-8 | Bio-CTGAGCCAGGATCAAACTCT |
| 1392R | 1392-1372 | ACGGGCGGTGTGTRC |
| Csp6l cas1 | NA | TTTGGATTTGCTGGTCGAATTCAACTA GGCTTAATCCGACG |
| Csp6l cas2 | NA | TACGTCGGATTAAGCCTAGTTGAATT |
| Deg cas1 | NA | Pho-TTTGTACGGCGGAGACGTCCGCCA CTAGTGTCGCAACTGACTA-AmMC7 |
| Deg cas2 | NA | TAGTCAGTTGCGACACTAGTGGCGGAC GTCTCCGCCGTACAAANN |
| GST1 | NA | GGATTTGCTGGTCGAATTCAAC |
| GST2 | NA | TAGTCAGTTGCGACACTAGTGGC |
a
Abbreviations: Pho, 5′ phosphate; AmMC7, 3′ amino modification; Bio, 5′ biotin; NA, not applicable.
b
E. coli numbering.