TABLE 6.

16S SP-GST identifier tags obtained from a microbial consortium comprised of D. radiodurans R1, B. licheniformis B-6-4J, A. globiformis DSM 20124, and the P. stutzeri strains Stanier 221 and BRW1^a

Sequence 5′→3′	Species	No. of occurrences	Tag no.	Upstream distance (bp)	Copy no.
GTACTATTTCTGAGCCTCGA	D. radiodurans	53	GST-DR1	238	2
GTACAGCGAGGAATGGCTCA	D. radiodurans	29	GST-MP1	26	1c
GTACGGCGCGGACGCTCTGC	D. radiodurans	26	GST-DR2	379	1
GTACATGCAAGTGTGCGTAG	B. licheniformis	46	GST-BL1	79	2
GTACATGCGAATGTGCGTAG	B. licheniformis	40	GST-BL2	79	2
GTACCTGTTAATTCATTTTT	B. licheniformis	28	GST-BL3	107	1
GTACCTGTTAATTCATTATA	B. licheniformis	28	GST-BL4	104	1
GTACCTGTTAATTCATTAAA	B. licheniformis	24	GST-BL5	44	1
GTACCGGCGCGGTGATAGAG	P. stutzeri	19	GST-PS1	450	2
GTACGGCGCAGGAGCGCGAT	P. stutzeri	11	GST-PS2	750	1
GTACGCGAAAGAACAAAGTT	P. stutzeri	7	GST-PS3	600	1
GTACGGCCAGCCTTCCCAGT	P. stutzeri	7	GST-PS4	1,200	1
GTACAAGTCCACGCCGGCAC	A. globiformisb	16	GST-AG1	930	8
GTACGTGTCGACGACCGGGG	A. globiformis	2	GST-AG2	1,236	4
GTACTGCACCCGGGAGGGTG	A. globiformis	NDd	GST-AG3	1,105	2
GTACTGCCGCCGAGCGGGGT	A. globiformis	ND	GST-AG4	1,236	1

^aTags were obtained using the 27R primer against position 8-27 (Fig. 1) of the 16S rRNA gene (1) and Csp6I (GTAC) as anchoring enzyme. For each tag, its occurrence in the sequenced tag library, its distance to the 16S rRNA gene from which it was derived, and copy number in its host strain are indicated. The tag copy numbers were confirmed by qRT-PCR. For both P. stutzeri strains, Stanier 221 and BRW1, identical qRT-PCR results were obtained.

^bFor A. globiformis DSM 20124, GST-AG3 and GST-AG4 were only found after conducting SP-GST analysis specifically on purified A. globiformis DNA, after which qRT-PCR was used to determine their respective copy numbers.

^cOn plasmid.

^dND, not detected.