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. 2006 Mar;72(3):1739–1748. doi: 10.1128/AEM.72.3.1739-1748.2006

FIG. 1.

FIG. 1.

Plasmid construct and predicted outcome of pΔsedA integration event. A 1,027-bp internal PCR fragment of sedA was cloned into the pAN7.1 plasmid (29). pAN7.1 carries the hygromycin resistance gene (HPH) from Escherichia coli as a dominant selectable marker under the control of the GPD promoter (pGPD) and the TRPC terminator (tTRPC) from Aspergillus nidulans. Homologous recombination of the plasmid construct with sedA by a single-crossover event results in the generation of two incomplete copies of the A. fumigatus gene [labeled sedA (N-term) and sedA (C-term)] separated by the linearized sequence of pAN7.1. The primers used to screen for the incomplete sedA genes are s1-A and s1-B (3′ fragment) and s1-C and s1-D (5′ fragment). For further information on primers, see Table 1.