. 2006 Mar;72(3):2272–2279. doi: 10.1128/AEM.72.3.2272-2279.2006

TABLE 1.

Strains and plasmids

Strain or plasmid	Replicon	Genotype, plasmid characteristic(s), and/or cloned cassette characteristic(s)	Reference or source
Strains
E. coli TG1		supE hsd Δ5 thi Δ(lac-proAB, F′ (traD36 proAB-lacZΔM15)	13
L. lactis MG1363		Wild type, plasmid free	12
L. lactis NZ9000		MG1363 (nisRK genes into the chromosome), plasmid free	14
Plasmids
pBS SK+II	ColE1	Ap^r	Stratagene
pIL252	pAMβ1	EmR, low copy number	28
pNucori1	ColE1 and pAMβ1	Ap^r/Em^r; high copy number; promoterless nuc gene	This work
pNucori2	ColE1 and pAMβ1	Ap^r/Em^r; low-copy number; promoterless nuc gene	This work
pSEC:NucT	pWV01	Cm^r; gene encodes SP_Usp-NucT precursor expressed under P_nisA	19
pSecDF	ColE1	Ap^r; carries the B. subtilis secDF gene	2
pSecDFh	ColE1 and pAMβ1	Ap^r/Em^r; high copy number	This work
pSecDF1	ColE1 and pAMβ1	Ap^r/Em^r; low copy number	This work
pSEC-L7/L12	pWV01	Cm^r; gene expressed under P_nisA encodes a SP_Usp-L7/L12 precursor	25
pVE3537	ColE1	ApR; carries promoterless nuc gene	19
pVE3556	PAMβ1	Em^r, derivative of high-copy-number plasmid pIL253	28
pVE3844	ColE1 and pAMβ1	Ap^r/Em^r; low copy number, encodes SecDF-cMyc expressed under P₅₉	This work
pVE5529	ColE1 and pAMβ1	Ap^r/Em^r; low copy number, encodes NucA expressed under P₅₉	8