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Trehalose biosynthesis from maltoheptaose in vitro. Trehalose synthesis was monitored by using cell extracts from C. glutamicum pJCtreZ (□), C. glutamicum pJCtreYZ (○), and a mixture of both cell extracts (▵). No significant trehalose synthesis was observed in cell extracts from the wild-type (×), C. glutamicum pJCtreY (•), and C. glutamicum ΔtreY (⋄). Each value represents the mean of three different uptake experiments ± the standard error.
