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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of dissolved proteins of E. coli BL21(DE3)pLysS cells synthesizing different BphA1 subunits. Contents of the lanes are indicated at the top. M, marker proteins; R, negative control cells harboring the vector pT7-6; 1, WT BphA; other numbers refer to the respective BphA1-MZ variants (see Fig. 5).
