TABLE 3.

Quantitation of productive CB dioxygenation by WT and variant BphAs

CB substrate	BphA variant	Amt of HOPDA ± SD (mA)a
4,4′	LB400	<5
	MZ7	93 ± 30
	MZ8	75 ± 11
	MZ11	198 ± 59
	MZ12	68 ± 21
3,4,4′	LB400	<5
	MZ7	1,060 ± 270
	MZ8	110 ± 20
	MZ11	822 ± 239
3,5,4′	LB400	<5
	MZ2	61 ± 10
	MZ4	515 ± 131
	MZ9	91 ± 27
	MZ14	50 ± 15
2,6,4′	LB400	135 ± 39
	MZ5	640 ± 181
	MZ13	509 ± 149

^aAbsorbance was recorded at λ_max (the wavelength at the absorption maximum) of the respective HOPDA (see Table 4).