TABLE 3.
Sensitivity of detection for V. parahamolyticus pure cultures using multiplexed real-time PCR with TaqMan probesa
| Sampleb | V. parahaemolyticus concn (CFU/ml) |
Ct value
|
|||
|---|---|---|---|---|---|
| tlh | ORF8 | tdh | trh | ||
| 1 | 107 | 17.25 ± 1.45 | 16.24 ± 3.70 | 14.73 ± 0.44 | 16.12 ± 0.24 |
| 2 | 106 | 19.96 ± 0.40 | 20.14 ± 0.36 | 18.61 ± 0.10 | 20.14 ± 0.08 |
| 3 | 105 | 23.53 ± 0.26 | 24.14 ± 0.85 | 22.21 ± 0.20 | 23.30 ± 0.06 |
| 4 | 104 | 28.29 ± 0.29 | 28.92 ± 1.09 | 25.95 ± 0.48 | 27.18 ± 0.26 |
| 5 | 103 | NDc | ND | 30.16 ± 0.41 | 30.47 ± 0.13 |
| 6 | 102 | ND | ND | ND | ND |
| 7 | 101 | ND | ND | ND | ND |
| 8 | 100 | ND | ND | ND | ND |
| 9 | Positive controld | 18.44 ± 0.58 | 18.60 ± 0.49 | 17.48 ± 0.25 | 19.11 ± 0.41 |
| 10 | Negative controle | ND | ND | ND | ND |
a
The data are means±standard deviations for three independent experiments.
b
The sample numbers correspond to the lane numbers in Fig. 1E.
c
ND, not detected.
d
The positive control included 100 ng purified V. parahaemolyticus DNA.
e
The negative control included 3 μl extract of T1N1 growth medium with no added cells.