TABLE 6.

Detection of V. parahaemolyticus in seeded oysters with overnight enrichment^a

Amt of V. parahaemolyticus seeded (CFU)	Final V. parahamolyticus concn (109 CFU/ml)b	Ct value
		tlh	ORF8	tdh	trh
105	3.88	18.94 ± 1.38	17.87 ± 2.03	16.75 ± 1.41	18.18 ± 1.57
104	4.20	19.33 ± 0.85	19.55 ± 0.59	17.24 ± 0.84	18.57 ± 1.22
103	2.80	19.36 ± 0.75	24.37 ± 2.26	17.55 ± 0.92	18.16 ± 1.03
102	4.65	20.08 ± 0.49	19.02 ± 0.97	17.40 ± 0.70	19.54 ± 0.93
101	4.00	19.55 ± 0.67	18.38 ± 0.86	17.18 ± 0.88	19.56 ± 1.31
100	3.30	20.84 ± 0.93	19.71 ± 0.50	18.61 ± 0.95	19.21 ± 1.44
0	NDc	NDc	ND	ND	ND
Positive controld		18.98 ± 0.42	18.16 ± 0.40	17.23 ± 0.40	19.29 ± 0.41
Negative controle		ND	ND	ND	ND

^aThe data are means ± standard deviations for four independent experiments.

^bThe viable plate count is an average for three dilutions.

^cND, not detected.

^dThe positive amplification control included 100 ng purified V. parahaemolyticus genomic DNA.

^eThe negative amplification control included 3 μl sterile water.