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Assessment of PhoB and the Pst system as regulators of Pho regulon expression. Phosphatase activities expressed as RFU normalized to the OD600 are shown for the WT and ΔphoB, Δpst, and Δpst phoB mutants grown under (A) Pi-limiting conditions (K10Tπ) and (B) Pi-sufficient conditions (K10T-1) for 6 h. Standard errors based on four replicates are shown.
