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The contribution of PhoD to Pho-regulated phosphatase activity. Shown are phosphatase activities expressed as RFU/OD600 unit for the ΔphoD mutant and the phoX phoD double mutant compared to the WT and the ΔphoB and phoX mutants. The strains were grown in Pi-limiting media (K10Tπ) for 6 h. The standard error was calculated based on four replicates.
