TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Reference |
|---|---|---|
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169(φ80lacZΔM15) hsdR17 thi-1 relA1 recA1 | 15 |
| S17-1(λpir) | thi pro hsdR hsdM+ ΔrecA RP4-2::TcMu-Km::Tn7 | 49 |
| Pseudomonas fluorescens | ||
| Pf0-1 | Wild type | |
| Δpst | Pf0-1 with deletion of pstSCAB-phoU; Gmr | This study |
| ΔphoB | Pf0-1 with deletion of phoB; Gmr | This study |
| Δpst phoB | Δpst with unmarked deletion of phoB; Gmr | This study |
| ΔphoD | Pf0-1 with deletion of phoD; Gmr | This study |
| PF-B6136 | Pf0-1; tatA::mini-Tn5lacZ1Km; Kmr | This study |
| PF-E280 | Pf0-1; tatC::mini-TnM; Gmr | This study |
| PF-E313 | Pf0-1; phoX::mini-Tn5lacZ1Km; Kmr | This study |
| PF-E313 ΔphoD | PF-E313 with deletion of phoD; Kmr Gmr | This study |
| PF-H1190 | Pf0-1; phoR::mini-Tn5lacZ1Km; Kmr | This study |
| PF-B337 | Pf0-1; apaH::mini-Tn5lacZ1Km; Kmr | This study |
| PF-B582 | Pf0-1; pvdL::mini-TnM; Gmr | This study |
| PF-apaH | Pf0-1::pKO-apaH; Tetr | This study |
| Plasmids | ||
| pAC-Ω-GM | Source of Gm cassette | 45 |
| pBBRMCS-5 | Broad-host range cloning vector; Gmr | 24 |
| pBB-apaGH | pBBRMCS-5 expressing apaGH | This study |
| pBT20 | Mini-TnM delivery vector; Apr Gmr | 26 |
| pEX18Tc | Positive selection knockout vector; sacB Tetr | 16 |
| pEX-phoB-Gm | pEX18Tc-derived vector for deletion of phoB; Tetr Gmr | This study |
| pEX-phoB | pEX18Tc-derived vector for unmarked deletion of phoB; Tetr | This study |
| pEX-pst-Gm | pEX18Tc-derived vector for deletion of pstSCABphoU; Tetr Gmr | This study |
| pEX-phoD-Gm | pEX18Tc-derived vector for deletion of phoD; Tetr Gmr | This study |
| pMini-CTX-lacZ | Site-specific integration vector; Tetr | 17 |
| pKO3 | Single-crossover knockout vector derived from pMini-CTX-lacZ | This study |
| pKO-apaH | pKO3 containing internal apaH fragment; Tetr | This study |
| pJB785TTKm1 | RK2 origin vector for luciferase transcriptional fusions; Kmr | 44 |
| pME3280a | Tn7 cloning vector; Apr Gmr | 65 |
| pTn7-PphoD-luc | Tn7 delivery vector for PphoD luciferase fusion; Apr Kmr | This study |
| pTn7-PphoD-Δbox | Tn7 delivery vector for PphoD-Δbox luciferase fusion; Apr Kmr | This study |
| pTn7-Ppst-luc | Tn7 delivery vector for Ppst luciferase fusion; Apr Kmr | This study |
| pUC-lucK | Derivative of pJB785TTKm with colE1 origin; Kmr | This study |
| pUC-Ppst-luc | pUC-lucK with Ppst luciferase fusion; Kmr | This study |
| pUC-PphoD-luc | pUC-lucK with PphoD luciferase fusion; Kmr | This study |
| pUC-PphoD-Δbox | pUC-lucK with PphoD luciferase fusion with mutated Pho box; Kmr | This study |
| pUC-PphoX-luc | pUC-lucK with PphoX luciferase fusion; Kmr | This study |
| pUC19 | colE1-based cloning vector; Apr | 64 |
| pUT-miniTn5-lacZ1-Km | Tn5 delivery vector; Apr Kmr | 7 |
| pUX-BF13 | Tn7 helper plasmid encoding Tn7 transposition functions; Apr | 65 |