TABLE 1.
CDSs included in the transcriptional survey
| CDSa | Predicted product | Transcriptional activity |
|---|---|---|
| pBt011 | Putative DNA-binding protein | Yes |
| pBt014 | Probable transcriptional regulator | Yes |
| pBt020 | Hypothetical protein | No |
| pBt021 | Cyt1Aa toxin | Yes |
| pBt023 | Cry11Aa crystal toxin | Yes |
| pBt029 | Putative DNA-binding protein | No |
| pBt031 | Putative N-acetylmuramoyl-l-alanine amidase (peptidoglycan hydrolase) | Yes |
| pBt036 | Cyt2Ba cytolytic delta-endotoxin | Yes |
| pBt054 | Cyt1Ca possible two-domain toxin | Yes |
| pBt056 | Hypothetical protein (potential pseudogene; matches pBt152 with two frameshifts and an in-frame stop) | No |
| pBt060 | Putative spore germination protein (pseudogene; contains two potential frameshifts) | No |
| pBt063 | Putative spore germination protein (pseudogene; truncated by IS240 insertion) | No |
| pBt075 | Hypothetical protein; weakly similar to a Yersinia pestis plasmid hypothetical protein | Yes |
| pBt084 | Putative spore germination protein; similar to B. subtilis GerAC | Yes |
| pBt085 | Putative spore germination protein; similar to B. subtilis GerBB | Yes |
| pBt086 | Putative spore germination protein; similar to B. subtilis GerKA | Yes |
| pBt087 | Putative 1-phosphatidylinositol phosphodiesterase precursor; contains an in-frame TGA stop after amino acid 86 | No |
| pBt091 | Putative transcriptional regulator, ArsR family | Yes |
| pBt092 | Small DNA-binding protein (bacterial histone-like family) | Yes |
| pBt093 | HfQ protein (RNA-binding protein) | No |
| pBt094 | Putative transcriptional regulator; similar to B. subtilis transition state regulators | Yes |
| pBt97 | Putative class II aminotransferase | Yes |
| pBt98 | Pyridoxal phosphate-dependent enzyme | Yes |
| pBt100 | tRNA synthetase-related protein | Yes |
| pBt101 | Possible kinase | Yes |
| pBt102 | GntR family transcriptional regulator containing aminotransferase domain | N/Db |
| pBt108 | Putative sigma factor, ECF family | Yes |
| pBt120 | Putative DNA-binding protein | Yes |
| pBt131 | Putative ABC transporter permease protein | No |
| pBt132 | Putative ABC transporter ATP-binding protein | N/D |
| pBt133 | Putative ABC transporter exported solute-binding protein | N/D |
| pBt136 | Possible peptide antibiotic precursor | Yes |
| pBt137 | Integral membrane protein (possible peptide antibiotic maturation and biosynthesis protein) | Yes |
| pBt138 | Integral membrane protein (possible accessory factor in peptide antibiotic secretion) | Yes |
| pBt139 | Putative ABC transporter ATP-binding protein | No |
| pBt145 | Putative spore coat-associated protein; similar to B. subtilis spore coat-associated protein N CotN | Yes |
| pBt147 | HfQ protein (RNA-binding protein) | No |
| pBt148 | Putative transcriptional regulator; similar to B. subtilis transition state regulators | Yes |
| pBt149 | Putative transcriptional regulator, ArsR family | Yes |
| pBt152 | Hemagglutinin-related protein | Yes |
| pBt156 | FtsZ/tubulin-related protein | Yes |
| pBt157 | Putative DNA-binding protein; contains predicted helix-turn-helix motif | Yes |
| pBt158 | Putative transcriptional regulator, MerR family | No |
a
The CDS designations are those described by Berry et al. (3), who describe each CDS and its database homologs in more detail. The transcriptional activity determined in this study is indicated.
b
N/D, not determined (activity of the gene was not assessed due to the presence of closely related genomic copies of the gene).