TABLE 2.
Annotation of genes involved in 4-chloronitrobenzene and nitrobenzene degradation and some properties of their encoded proteins
| Gene | Position in sequence (bp) | Gene product | Calculated mass, Da (no. of residues) | Homologous protein | Source | % Identity/no. of residues | Accession no. |
|---|---|---|---|---|---|---|---|
| cnbR | 330-845 | Regulatory protein | 18,761 (171) | NbzR | P. putida HS12/pNB1 | 64/114 | AAK26517 |
| MarR | Polaromonas sp. strain JS666 | 38/100 | EAM40420 | ||||
| orf1 | 1131-1550 | Putative ferredoxin | 15,130 (139) | ORF1 | P. pseudoalcaligenes JS45 | 57/125 | AAF03490 |
| NbzJ | P. putida HS12/pNB1 | 56/125 | AAK26518 | ||||
| NbzCa | P. putida HS12/pNB1 | 79/300 | AAK26519 | ||||
| cnbCa | 1623-2561 | 2-Aminophenol-1,6-dioxygenase | 35,037 (312) | AmnB | P. pseudoalcaligenes JS45 | 78/300 | AAB71524 |
| beta subunit | AmnB | Pseudomonas sp. strain AP-3 | 80/295 | BAB03531 | |||
| AmnA | P. pseudoalcaligenes JS45 | 60/271 | AAB71525 | ||||
| cnbCb | 2574-3389 | 2-Aminophenol-1,6-dioxygenase | 29,264 (271) | NbzCb | P. putida HS12/pNB1 | 60/271 | AAK26520 |
| alpha subunit | AmnA | Pseudomonas sp. strain AP-3 | 58/271 | BAB03532 | |||
| AmnC | Pseudomonas sp. strain AP-3 | 75/488 | BAB03533 | ||||
| cnbD | 4581-6053 | 2-Aminomuconic 6-semialdehyde | 54,026 (490) | NbzD | P. putida HS12/pNB1 | 75/487 | AAK26521 |
| dehydrogenase | AmnC | P. pseudoalcaligenes JS45 | 74/320 | AAC33839 | |||
| cnbE | 6197-7036 | 2-Keto-4-pentenoate hydratase | 29,781 (279) | TdnG | P. putida UCC22 | 65/261 | BAB62054 |
| cnbF | 7056-7844 | 4-Oxalocrotonate decarboxylase | 28,077 (262) | CdoK | Comamonas sp. strain JS765 | 69/227 | AAG17138 |
| AphH | Comamonas testosteroni TA441 | 69/227 | BAA88505 | ||||
| cnbG | 8097-8288 | 4-Oxalocrotonate tautomerase | 7,158 (63) | AphI | Comamonas testosteroni TA441 | 76/63 | BAA88507 |
| cnbH | 8728-10011 | 2-Amino-5-chloromuconic acid deaminase | 44,966 (427) | GatA | Agrobacterium tumefaciens C58 | 38/371 | NP_533743 |
| cnbI | 10054-10569 | 2-Oxopent-4-dienoate hydratase | 18,459 (271) | CbzJ | P. putida GJ31 | 98/165 | AAX38586 |
| TdnG | P. putida UCC22 | 37/166 | BAB62054 | ||||
| cnbA | 1869-2552 | 4-Chloronitrobenzene | 25,967 (227) | NbzA | P. putida HS12/pNB1 | 92/227 | AAK26512 |
| nitroreductase | NbzA | P. pseudoalcaligenes JS45 | 88/227 | AAT71308 | |||
| cnbB | 281-733 | Hydroxylaminobenzene mutase | 15,951 (150) | HabB | P. pseudoalcaligenes JS45 | 51/115 | AAB94123 |
| NbzB | P. putida HS12/pNB2 | 50/115 | AAK26516 |