FIG. 3.

Expression of a chromosomal catB::lacZ fusion in strains encoding CatM or variant regulators [CatM(V158M), encoded by catM5153, or CatM(R156H), encoded by catM3102]. All strains lack a functional benM. Cultures were grown on succinate with or without muconate as an inducer, as indicated. β-Galactosidase (LacZ) activity is shown relative to that measured for succinate-grown ACN585 (3.96 μmol/min/ml/OD₆₀₀). Activities are averages of at least three repetitions, and standard deviations were <20% of the average value.