FIG. 3.

Metabolite accumulation in cultures of P. aeruginosa grown in citronellol. Cultures were grown in M9 medium with succinic acid as the carbon source for 18 h, and citronellol was then added. The metabolites were extracted from the supernatants and analyzed at 48 h after the addition of citronellol by gas chromatography as described in Materials and Methods. Commercial citral (10.7 and 11.4 min), citronellol (11.6 min), citronellic acid (20.4 min), and geranic acid mix isomers (21.3 and 22.2 min) were used as standard compounds. Peaks with retention times of 20.4 and 21.3 min correspond to citronellic acid and geranic acid, respectively.