FIG. 5.

Induction of the liuB::lacZ transcriptional fusion. Cultures of the PAO1 strain with plasmid pANP2 (P2liuB::lacZ) were grown in M9 medium with glucose. β-Galactosidase activity was measured after the addition of the inducer compounds glucose (0.2%), leucine (0.005%), 3-methylcrotonoic acid (0.005%), and citronellol (0.005%). Data given correspond to Miller units after 3 h of induction with the compound indicated and are the averages of two independent experiments done in duplicate; the standard deviation was less than 5% of the given value.