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. 2006 Mar;72(3):2070–2079. doi: 10.1128/AEM.72.3.2070-2079.2006

FIG. 6.

FIG. 6.

Phylogenetic trees of AtuF/LiuD protein orthologs. Trees were obtained by the UPGMA, NJ, and ME methods in the Mega2 package as described in Materials and Methods, and the NJ tree is shown. (A) The tree shows the five phylogenetic groups of the biotinylated subunits of acyl-CoA carboxylases aligned; the probable functions of the ortholog proteins are indicated in the phylogenetic groups, in agreement with some characterized carboxylases: acetyl-CoA carboxylases (blue), propionyl-CoA carboxylases (orange), 3-methylcrotonyl-CoA carboxylases (green), pyruvate carboxylases (red), and urea carboxylases (yellow). Acyl-CoA carboxylases from P. aeruginosa PAO1 are shown by arrows. (B) Phylogenetic tree of MCCase root constructed with the 41 proteins showing the higher phylogenetic relationship. The AtuF and LiuD proteins of P. aeruginosa are shown by arrows. Bootstrap values of higher than 50% obtained by the UPGMA, NJ, and ME methods are shown. Blue, Alphaproteobacteria; purple, Betaproteobacteria; green, Gammaproteobacteria; yellow, Actinobacteria; orange, Bacillus; gray, Spirochaeta; red, eukaryote.