TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| P. aeruginosa PAO1SM | Spontaneous streptomycin-resistant mutant derived from strain PAO1 | 33 |
| P. fluorescens L1 | Environmental strain | Donated by E. Valencia |
| P. putida | Environmental strain | Donated by S. Aguilera |
| PAO atuC | PAO1::ISlacZ/hah (ID 10287), transposon insertion in ORF PA2888 | 13 |
| PAO atuD | PAO1::ISlacZ/hah (ID 15357), transposon insertion in ORF PA2889 | 13 |
| PAO atuE | PAO1::ISlacZ/hah (ID 8388), transposon insertion in ORF PA2890 | 13 |
| PAO atuF | PAO1::ISlacZ/hah (ID 10788), transposon insertion in ORF PA2891 | 13 |
| PAO2893 | PAO1::ISlacZ/hah (ID 8377), transposon insertion in ORF PA2893 | 13 |
| PAO liuD | PAO1::ISlacZ/hah (ID 11254), transposon insertion in ORF PA2012 | 13 |
| PAM1529 (atuF) | PAO1SM mutant obtained by Himar1::Gmr transposition in ORF PA2891, unable to grow on citronellol | This work |
| PAE80 (liuD) | PAO1SM mutant obtained by Himar1::Gmr transposition in ORF PA2012, unable to grow on citronellol | 5 |
| PAO atuF liuD | Double mutant strain derived from PAO atuF, mutated in liuD gene | This work |
| PAO atuF liuB | Double mutant strain derived from PAO atuF, mutated in liuB gene | This work |
| PAO atuF liuE | Double mutant strain derived from PAO atuF, mutated in liuE gene | This work |
| PAO atuC liuE | Double mutant strain derived from PAO atuC, mutated in liuE gene | This work |
| PAM liuA | PAO1SM liuA::Gmr mutant obtained by recombination in ORF PA2015 | 5 |
| PAM liuB | PAO1SM liuB::Gmr mutant obtained by recombination in ORF PA2014 | 5 |
| PAM liuD | PAO1SM liuD::Gmr mutant obtained by recombination in ORF PA2012 | 5 |
| PAM liuE | PAO1SM liuE::Gmr mutant obtained by recombination in ORF PA2011 | 5 |
| Plasmids | ||
| pUCP20 | pUC19-derived E. coli-Pseudomonas shuttle vector; Apr Cbr | 32 |
| pMO013850 | pLA2917 cosmid plus 25 kb of PAO1 genome, including the liu cluster | Pseudomonas Genetic Stock Center |
| pMO011609 | pLA2917 cosmid plus 25 kb of PAO1 genome, including the atu cluster | Pseudomonas Genetic Stock Center |
| pAL-22 | pUCP20 with 7.42-kb HindIII-EcoRV DNA fragment containing liuABCDE genes from pMO013850 cosmid | 5 |
| pAL-23 | pUCP20 with 7.84-kb KpnI-XbaI DNA fragment containing atuCDEF genes from pMO011609 cosmid | This work |
| pLP170 | lacZ transcriptional fusion vector able to replicate in P. aeruginosa | 23 |
| pANP2 | P2 liuB::lacZ fusion derived from pLP170 | 5 |