TABLE 2.
Growth phenotypes of Pseudomonas strains
| Strain | Growth ina:
|
Doubling time (h)e | ||
|---|---|---|---|---|
| Citronellol | l-Leucine | l-Isoleucine | ||
| P. aeruginosa PAO1SM | +++ | +++ | +++ | 8.2 |
| P. fluorescens L1 | − | +++ | +++ | NDf |
| P. putida | − | +++ | +++ | ND |
| PAO2893b | +++ | +++ | +++ | 8.0 |
| PAO atuDb | − | +++ | +++ | 7.5 |
| PAO atuEb | − | +++ | +++ | 11.2 |
| PAO atuCb | − | ++ | +++ | 13.1 |
| PAO atuFb | − | + | +++ | 13.5 |
| PAO atuF(pAL-22)b | − | ++ | +++ | 6.5 |
| PAO atuF(pAL-23)b | +++ | +++ | +++ | 7.0 |
| PAM1529 (atuF mutant)c | − | +++ | +++ | 13.1 |
| PAM1529(pAL-22)c | − | +++ | +++ | 6.5 |
| PAM1529(pAL-23)c | +++ | +++ | +++ | 6.5 |
| PAE80 (liuD mutant)c | − | − | +++ | ND |
| PAE80(pAL-22)c | +++ | +++ | +++ | 9.0 |
| PAE80(pAL-23)c | − | − | +++ | ND |
| PAM liuDd | − | − | +++ | ND |
| PAM liuD(pAL-22)d | +++ | +++ | +++ | 9.7 |
| PAM liuD(pAL-23)d | − | − | +++ | ND |
| PAO liuDb | − | +++ | +++ | ND |
| PAM liuAd | − | − | +++ | ND |
| PAM liuBd | − | − | +++ | ND |
| PAM liuEd | − | − | +++ | ND |
| PAO atuF liuD | − | − | +++ | ND |
| PAO atuF liuB | − | − | +++ | ND |
| PAO atuF liuE | − | − | + | ND |
| PAO atuC liuE | − | − | + | ND |
The strains were inoculated on M9 plates with the compounds indicated as sole carbon sources and incubated for 48 h at 30°C as described in Materials and Methods. +++, good growth; ++, medium growth; +, low growth; −, no growth.
Mutant obtained by Jacobs et al. (13) (no polar effects over downstream gene expression).
Mutant described in this work (polar effects over downstream gene expression).
Mutant obtained by homologous recombination (polar effects over downstream gene expression).
Doubling times of cultures grown in M9 minimal liquid medium with leucine (0.2%, wt/vol) as the sole carbon source, incubated at 30°C with shaking at 150 rpm. The growth was measured by optical density at 600 nm and calculated in the exponential growth phase.
ND, not determined.