TABLE 3.

Carboxylase activity in P. aeruginosa PAO1 cell extracts

Carbon sourceb	Carboxylase activitya (U/mg of protein) on substrate:
	3-Methylcrotonyl-CoA	Acetyl-CoA	Propionyl-CoA
Glucose	82.0	36.2	47.8
Glucose + leucine	97.0	143.0	82.0
Glucose + citronellol	45.1	50.0	44.0
Leucine	492.0	NDc	ND
Citronellol	1,312.0	73.8	66.9

^aEnzymatic quantification was carried out as described in Materials and Methods. One unit of enzyme activity catalyzed the incorporation of 1 μmol of ¹⁴C into acid-stable product/min. Data given are the averages of two quantifications in duplicate; the standard deviation was less than 10%.

^bCell extracts were grown on minimal medium M9 with the carbon source indicated.

^cND, not determined.