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. 2006 Mar;72(3):2235–2238. doi: 10.1128/AEM.72.3.2235-2238.2006

FIG. 1.

FIG. 1.

Scheme for plasmid pRCA construction and Southern blot analysis. (A) Map of recombinant plasmid pRCA. (B) Ethidium bromide-stained agarose gel of the genetically manipulated products. Lanes: M, HindIII-digested λ DNA molecular size markers; 1 and 4, PCR-amplified products of carA from strains XLDN4-9 and SN8, respectively; 2, native plasmid pRESQ; 3, recombinant plasmid pRCA. (C) Southern blot analysis of the total DNAs digested with HindIII from the XP (lane 5) negative control and the recombinant SN8 (lane 6) probed with the carAa gene.