FIG. 2.

Organization of the two TPA degradation gene clusters in Comamonas sp. strain E6 and deletion analysis. Restriction maps of the 7.3-kb SalI-HindIII fragment carrying the tph_I genes and the 11-kb SacI-HindIII fragment carrying the tph_II genes are shown. The lines above and below the maps indicate the region included in each plasmid. These plasmids (except pJSK432, which was used as a complementary plasmid for the tphA2_I tphA2_II double mutant) were introduced into C. testosteroni IAM 1152 to examine role of each tph gene. kan and cat indicate the positions of the kan and cat gene insertions into the tphA2_I mutant, the tphA2_II mutant, and the tphA2_I tphA2_II double mutant. Abbreviations for restriction enzymes: Bg, BglII; E, EcoRI; E47, Eco47III; H, HindIII; K, KpnI; M, MunI; N, NheI; P, PstI; Sc, SacI; Sl, SalI; Sm, SmaI; Sp, SphI; Tt, Tth111I; Xb, XbaI; Xh, XhoI.