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. 2006 Mar;72(3):1729–1738. doi: 10.1128/AEM.72.3.1729-1738.2006

FIG. 2.

FIG. 2.

CLUSTAL alignment of BSHs. Protein sequences were obtained from the National Center for Biotechnology Information site (http://www.ncbi.nlm.nih.gov/). Alignments were performed by using CLUSTAL W (http://www.ebi.ac.uk/clustalw/). Those amino acids thought to be involved in catalysis (Cys-1, Asp-21, Tyr-82, Asn-175, and Arg-228) are boxed. Identical amino acids are marked by an asterisk, conserved substitutions are marked by two dots, and semiconserved substitutions are marked by a single dot. BB, Bifidobacterium bifidum (AAT11513 [50]); BL, Bifidobacterium longum (AAF67801 [88]); CP, Clostridium perfringens (P54965 [14]); LA, Lactobacillus acidophilus (AAD03709 [31]); LJ, Lactobacillus johnsonii (AAG22541 [31]); LP, Lactobacillus plantarum (AAB24746 [13]); LM, Listeria monocytogenes (CAD00145 [5, 27]); and BS, Bacillus sphaericus (P12256 [70]).