Skip to main content
. Author manuscript; available in PMC: 2006 Mar 8.
Published in final edited form as: J Biol Chem. 2003 Sep 24;278(49):48580–48589. doi: 10.1074/jbc.M310301200

Figure 1.

Figure 1

Activation of wild-type and R198G mutant mesotrypsinogen (PRSS3) with bovine enterokinase. Panel A. Trypsinogen samples at 2 μM final concentration were incubated with 200 ng/mL enterokinase (final concentration) at 37 °C, in 0.1 M Tris-HCl (pH 8.0), 1 mM CaCl2 and 2 mg/mL bovine serum albumin in a final volume of 100 μL. Aliquots of 2.5 μL were withdrawn from reaction mixtures at indicated times and trypsin activity was determined with N-CBZ-Gly-Pro-Arg-p-nitroanilide. Trypsin activity was expressed as percentage of potential maximal activity. For comparison, activation of human cationic trypsinogen (PRSS1) and anionic trypsinogen (PRSS2) are also shown. Panels B and C. Reactions incubated without bovine serum albumin were terminated at indicated times by precipitation with 10 % trichloroacetic acid (final concentration), electrophoresed on SDS-polyacrylamide gels (13 %) under reducing conditions and stained with Coomassie blue.