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A C-Terminally Fused PSI Domain Causes ER Retention of GFP.
(A) Tobacco protoplasts expressing sGFP-HDEL. GFP labels cortical elements of the ER (left) and the nuclear envelope (right). Measurement of fluorescence intensity is restricted to the cell cortex and the nuclear envelope, as indicated by the line measurement. Magnification ×600.
(B) Tobacco protoplasts expressing sGFP-PSI show a similar distribution of fluorescence within the cell.
