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. 2001 Sep;13(9):2021–2032. doi: 10.1105/TPC.000533

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Copyright © 2001, American Society of Plant Biologists

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Figure 7. — PhytepsinΔPSI Is Secreted in a COPII-Independent Manner.

Secretion/retention experiment using transient expression to investigate the cellular localization of wild-type Phytepsin (WT) and PhytepsinΔPSI (ΔPSI) and the effect of increasing levels of Sec12p (Sec12). The secretion index was determined for the internal secretory marker α-amylase (Amy) by calculating the ratio between the extracellular levels and the intracellular levels of the enzyme (Phillipson et al., 2001) and is used to quantify the inhibition of COPII-mediated ER export. Values for the secretion indices are from single experiments corresponding to the same protoplast suspension analyzed in the protein gel blots at bottom. Secretion index values for the experiment with PhytepsinΔPSI are illustrated with white bars, and values for the wild type are illustrated with gray bars. Annotations are as in Figures 1 and 2, and the amounts of cotransfected plasmid encoding Sec12p are given in micrograms. Note that in contrast to the internal secretory marker α-amylase and wild-type Phytepsin (bottom), PhytepsinΔPSI secretion is not notably affected by Sec12p overexpression.