Figure 7.

DNA Binding Property of OSH15.

(A) Target sequences of TALE HD proteins that have been reported. Most TALE HD proteins share the same target sequence (i.e., the TGTCAC motif).

(B) The TGTCAC motif and its derivative sequences. Oligonucleotides containing these sequences were used in this study.

(C) Recombinant histidine-tagged OSH15 was subjected to EMSA using different DNA probes as indicated above each lane. Probe sequences are shown in (B).

(D) EMSA was performed using histidine-tagged OSH15 derivatives, as indicated above the gel lanes. Both the histidine tag and no protein were used as negative controls. The arrowheads indicate the positions of the faster mobility bands (lanes 6 and 7), in contrast to the shifted bands with slower mobility, which are indicated by arrows (lanes 2 and 3).