Figure 9.

Homodimer Formation of OSH15 in a Quantitative Yeast Two-Hybrid Assay.

Homodimer formation of OSH15 derivatives in yeast. Shown at left is the pairwise combination of OSH15 derivatives. The shaded bars show the relative LacZ activity of each pairwise combination of the OSH15 derivatives. The cross-hatched bars show the relative LacZ activity of GAL4-DB fused with each OSH15 derivative protein alone used as negative control. Error bars represent standard deviations. Relative LacZ activity was calculated by standardizing each LacZ activity with that of intact OSH15/OSH15. We set the activity of OSH15/OSH15 at 100.