Figure 1.

Immunoblot Analysis of Thylakoid Membrane Proteins from hcf107 and Wild Type.

The lanes were loaded with 10 μg (wild type [WT], hcf107-1, and hcf107-2), 5 μg (WT 1/2), 2.5 μg (WT 1/4), or 1.25 μg (WT 1/8) of total membrane proteins from ∼3-week-old plants grown on a sucrose-supplemented Gelrite medium (see Methods). Proteins were detected with the indicated antiserum to subunits of PSII, PSI, the cytochrome (Cyt) b₆f complex, and the chloroplast ATP synthase (ATPase) as described (Meurer et al., 1996b).