Figure 3.

Identification of the 8-kD Protein by Immunoblotting and in Vitro Phosphorylation Experiments.

(A) Immunoblot analysis with an antiserum to the PsbH subunit of PSII. The analysis was performed and labels are as described in the legend to Figure 1.

(B) In vitro phosphorylation with thylakoids of hcf107 (hcf107-1), hcf152, and wild-type (WT) seedlings. For experimental details, see Methods. Wild-type and mutant proteins with equivalent amounts of radioactivity (80,000 cpm) were separated electrophoretically on a 12.5% polyacrylamide-SDS/urea gel (Schägger and von Jagow, 1987) and analyzed by fluorography.