Figure 2.

Analysis of Photosynthetic Membrane Proteins from the Mutant hcf164 and the Wild-Type (WT).

Twenty micrograms (hcf164, WT1/1), 10 μg (WT1/2), 5 μg (WT1/4), and 2.5 μg (WT1/8) of chloroplast proteins were size separated by SDS-PAGE and electroblotted onto nitrocellulose membranes.

(A) Detection of the heme binding proteins cytochrome f and cytochrome b₆ by enhanced chemiluminescence.

(B) Immunoblot analysis of the cytochrome b₆f subunits cytochrome f, cytochrome b₆, the Rieske-FeS protein (Rieske), and PetD.

(C) Immunodetection of the subunits PsaA/B and PsaD of PSI, the inner antenna protein CP47, and the reaction center proteins D2 and cytochrome b₅₅₉ of PSII.

Cyt, cytochrome.