Figure 3.

In Vivo Protein Synthesis in hcf164 Mutants and Wild-Type (WT) Plants.

Primary leaves of 15-day-old plants were radiolabeled with ³⁵S-methionine for 30 min.

(A) Membrane proteins were isolated, separated by SDS-PAGE, and analyzed by autoradiography as described in Methods. Proteins equivalent to 100,000 incorporated cpm or the indicated dilutions of wild-type were loaded in each lane.

(B) Membrane proteins were solubilized, and the protein equivalent of 200,000 incorporated cpm was used for immunoprecipitation with antisera against cytochrome f (Cyt f) and the PetD subunit. Lanes WT and hcf164 were loaded with 50% of the appropriate immunoprecipitation reaction, and lanes WT1/2 and WT1/4 were loaded with 25 and 12.5% of the WT precipitation, respectively. For experimental details, see Methods.