Figure 8.

Lumenal Localization of the HCF164 Protein.

Intact and sonicated spinach thylakoids were protease treated with thermolysin (final concentration, 0.1 mg/mL). The reaction was stopped at the times indicated by the addition of EDTA to a final concentration of 50 mM. Membranes were recovered by centrifugation, and the equivalent of 10 μg of chlorophyll was loaded in each lane for immunoblot analysis. Ferredoxin NADP oxidoreductase (FNR) was used as a control for a peripheral thylakoid protein that is exposed to the stromal side, and the 23-kD protein of PSII was used as control for a lumenal protein.