Figure 1.

The Identity of Multimers Resulting from PCR Amplification of B2-Tourist Elements in Maize Line B79.

PCR products resolved by agarose gel electrophoresis and visualized by ethidium bromide staining were purified, cloned, and sequenced (see Methods). The annealing temperature for PCR was 60°C for the two samples at left and 55°C for the three samples at right. The identities of PCR bands are diagrammed at right. The positions of the insertion sites of the various Tourist subfamily members (Zm3, Zm11, Zm22, and Zm29) (Bureau and Wessler, 1992, 1994a; N. Jiang and S. Wessler, unpublished data) into B2 elements are shown along with the TSD. M, DNA molecular weight standard (bp).