Table 1.
Maize Line | Preexisting Element | Insertion Element | Insertion Sitea (bp) | TSD | Source |
---|---|---|---|---|---|
B79 | B2 | Zm3 | 37 | TGA | B2 PCR |
B79 | B2 | Zm11 | 37 | TAA | B2 PCR |
B79 | B2 | Zm29 | 37 | TGA | B2 PCR |
B79 | B2 | Zm22 | 27 | TTA | B2 PCR |
B79 | B2b (1) | Zm3b (2) | 37 | TGA | B2 PCR |
B79 | Zmb (2) | Zm3b (3) | 47 | TTA | B2 PCR |
B73 | B2 | Zm3 | 27 | TTA | Two-step PCR |
RIL22c | Zm13 | Zm3 | 46 | TAA | Two-step PCR |
B73 | B2 | Zm3 | 37 | TTA | Two-step PCR |
B73 | Zm3 | Zm3 | 47 | TAA | Two-step PCR |
B73 | Zm3 | Zm3 | 57 | TTA | Two-step PCR |
B73 | Zm13 | Zm3 | 67 | TTA | Two-step PCR |
B73 | Zm24 | Zm3 | 47 | TCA | Two-step PCR |
B79 | B2 | Zm3 | 37 | TGA | Two-step PCR |
RIL7 | Zm24 | Zm3 | 37 | TGA | Two-step PCR |
Spanco | Zm13 | Zm22 | 67 | TTA | Two-step PCR |
Spanco | Zm24 | Zm22 | 59 | TAA | Two-step PCR |
B79 | B2 | Zm28 | 58 | TAA | Genomic library |
B79 | Zm13 | Zm25 | 46 | TGA | Genomic library |
B79 | Zm27b (1) | Zm3b (2) | 47 | TGA | Genomic library |
B79 | Zm3b (2) | Unknown elementb (3) | 47 | TAA | Genomic library |
Insertion site is defined as the number of base pairs from the closest end of the target element to the first nucleotide of the TIR of the insertion element.
Elements that form trimers. The numbers in parentheses indicate the order of insertion. Element 1 is the first preexisting element; element 2 is the element that inserted into element 1; element 3 is the element that inserted into element 2.
RIL represents recombinant inbred lines from the B73 × Mo17 cross.