Effects of ESR1 Overexpression on Shoot Regeneration from Root Explants.
(A) Representative plates 4 weeks after transformation. Arabidopsis root cultures transformed with the vector alone (pER10) or pER10-ESR1 were cultured on SIM with or without cytokinin (25 μM 2-ip) or an inducer of transcription (5 μM 17β-estradiol).
(B) Numbers of transformed shoots from 0.1 g fresh weight of root culture. Data represent the average of four independent experiments, and error bars indicate standard deviations. Open columns show transformants with the vector alone (pER10), and cross-hatched columns show transformants with pER10-ESR1.