Figure 3.

Sensitivity of ESR1-Overexpressing Cells to Cytokinin.

(A) Numbers of regenerated shoots in different concentrations of cytokinin. Ten root segments from transgenic seedlings carrying either pER8 or pER8-ESR1 were cultured on plates with MS medium supplemented with the indicated concentrations of 2-ip with or without an inducer (10 μM 17β-estradiol). Each value represents the average of three independent experiments, and bars indicate standard deviations.

(B) RNA gel blot analysis of the ESR1 transcript. Four micrograms of total RNA was loaded on each lane and hybridized with an ESR1 cDNA probe or a 25S rRNA probe.