Figure 4.

Effects of ESR1 Overexpression on the Formation of Calli, Shoots, and Roots.

(A) and (B) Root segments on RIM with (B) or without (A) inducer.

(C) and (D) Root segments on SIM with (D) or without (C) inducer.

(E) and (F) Root segments on CIM with (F) or without (E) inducer.

17β-Estradiol (10 μM) was used as an inducer in (B), (D), and (F). Root segments from a transgenic Arabidopsis line carrying a pER8-ESR1 construct were preincubated on CIM for 3 days and then transferred onto the media described above. Photographs were taken 4 weeks after transfer onto individual media. Bars = 0.5 cm.