Figure 5.

Inhibition of Shoot Regeneration Caused by Constitutive ESR1 Expression.

(A) Phenotype of transformants obtained by root transformation. Root segments were transformed with pSK-ESR1, a 35S:ESR1 construct, after incubation on CIM for 3 days and then transferred onto SIM containing kanamycin (50 mg/L) and carbenicillin (300 mg/L) for 4 weeks. Six representative explants are shown.

(B) Phenotype of seedling obtained by in planta transformation. Seedlings harboring a 35S:ESR1 construct were germinated on MS medium containing kanamycin and carbenicillin. The photograph was taken 6 weeks after germination.

(C) Magnified root part of (B).

(D) Magnified aerial part of (B).

Bars = 1 cm.