Figure 6.

Time Course of ESR1 Expression during Shoot Regeneration.

RNA gel blot analysis of the ESR1 transcript. Root segments were preincubated on CIM for 4 days and then transferred onto SIM. Total RNAs were prepared from root explants at the times indicated, and 10 μg of total RNA was loaded. RNA gel blots were hybridized to different cDNA probes. Numbers above the lanes indicate days (A) or hours (B) after transfer onto SIM. In (B), in the first lane from the right (CIM), after pretreatment on CIM, root segments were incubated on fresh CIM for 24 hr.