Skip to main content
. 2001 Dec;13(12):2631–2642. doi: 10.1105/tpc.010315

Figure 6.

Figure 6.

Complementation of agb1 and Identification of the Molecular Lesion in agb1-1.

(A) Comparison of phenotypic traits among wild-type plants (WT; Col ecotype), agb1-1, and complemented plants (homozygous agb1-1 transformed with pCAMBIA2300-T4L20.4). At top, rosette phenotypes of 3-week-old plants. Bar = 1 cm. At bottom, silique tip phenotypes from each genotype. Bar = 1 mm.

(B) At top, the AGB1 locus is comprised of six exons and five introns. Sequence analysis of agb1-1 genomic DNA and AGB1 cDNA from agb1-1 showed that the molecular lesion is a transition mutation altering the splicing donor site at the 5′ end of the first intron of AGB1. At bottom is the mature AGB1 mRNA from agb1-1.

(C) A portion of the AGB1 genomic DNA sequence flanking the first exon/intron junction, showing the molecular lesion in agb1-1. Above the exon DNA sequence, the amino acid residues for the last four entire codons before the first intron are shown.