Figure 2.

Expression Domains of SPAK, SIP4, and SPGB Overlap with That of SP.

(A) to (C) Localization of SPAK mRNA in the early (two-leaf stage) vegetative apex (A), the first sympodial apex and an early floral primordium (B), and a floral bud (C).

(D) and (E) Localization of SIP4 (D) and SPGB (E) mRNA in early vegetative apices.

(F) Control section displaying contrasting expression pattern of the gene for the small subunit of the tomato ribonucleotide-reductase small subunit gene (RNR2). RNR2 displays a discontinuous pattern, reflecting the distribution of cells in S phase.

Digoxygenin-labeled antisense RNA probes for in situ hybridizations were prepared from inserts cloned in the BS vector using the T7 polymerase. C, carpel; FP, floral primordium; L, leaf; P, petal; PR, primary apex; S, sepal; SA, sympodial apex; ST, stamen; VS, vascular strand.