Figure 5.
Light-Induced Accumulation of psbD-LRP Transcripts Is Reduced in phyA Mutants.
Primer extension analysis was used to monitor psbD-LRP (A) and rbcL (B) transcript levels in the wild type (Ler) and phyA mutants. Plants were dark-adapted (D) and then illuminated for 48 hr with blue (B) or red (R) light at 30 μE·m−2·sec−1.
(A) psbD transcripts derived from the psbD-LRP (950 nucleotides) are indicated by an arrow and labeled as such. Other psbD transcripts are designated by an arrow and labeled as psbD #2 and psbD #3. Lane φ shows φX174-HaeIII restriction fragments used as size markers.
(B) rbcL transcripts are indicated by an arrow and labeled.